Extracellular matrix of the human retinal pigment epithelial cells in vitro.

Ko,  Myung Kyoo; Choe,  Joon Kiu

doi:1988.2.2.66

Korean J Ophthalmol > Volume 2(2); 1988 > Article

Original Article

Korean Journal of Ophthalmology 1988;2(2):66-68.
DOI: https://doi.org/10.3341/kjo.1988.2.2.66 Published online December 30, 1988.

Extracellular matrix of the human retinal pigment epithelial cells in vitro.

Myung Kyoo Ko, Joon Kiu Choe

Department of Ophthalmology, School of Medicine, Hanyang University, Seoul, Korea.

Abstract

This study was undertaken to localize fibronectin and type IV collagen in the cultured retinal pigment epithelial cell by means of immunofluorescent staining and immunocytochemical methods. Immunofluorescent staining and immunocytochemical methods revealed fibronectin and type IV collagen localized on the extracellular membrane of the cultured retinal pigment epithelial cell. Ultrastructural immunocytochemical technique also revealed fibronectin associated with extracellular tissue. This study demonstrated that fibronectin and type IV collagen are an integral component of the extracellular matrix of the human retinal pigment epithelial cell in vitro.

Key Words: extracellular matrix;retinal pigment epithelium;fibronectin;type IV collagen