Ascorbic Acid Enhances Nitric Oxide Production in Trabecular Meshwork Cells. |
Jae Woo Kim |
Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
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Abstract |
PURPOSE This study investigated the role of ascorbic acid on the production of nitric oxide (NO) in the trabecular meshwork (TM) cells. METHODS: After primarily cultured human TM cells were exposed to 1, 10, and 100 micrometer of L-ascorbic acid (LAA), with or without co-administration of 1 mM sodium nitroprusside or 100 micrometer hydrogen peroxide for 48 hr, cellular survival and NO production were measured with MTT and Griess assay, respectively. RESULTS: LAA significantly potentiated NO production in a dose-dependent manner (p< 0.05) without affecting cell viability. LAA increased cell viability after hydrogen peroxide-induced oxidative stress in a dose-dependent manner. LAA enhanced NO production in TM cells and showed a cytoprotective effect against hydrogen peroxide-induced oxidative stress. CONCLUSIONS: LAA might be involved in the regulation of trabecular outflow by enhancing NO production in TM cells. |
Key Words:
Ascorbic acid;Hydrogen peroxide;Nitric oxide;Trabecular meshwork cell |
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