Korean J Ophthalmol > Volume 19(3); 2005 > Article
Korean Journal of Ophthalmology 2005;19(3):227-232.
DOI: https://doi.org/10.3341/kjo.2005.19.3.227    Published online September 30, 2005.
Ascorbic Acid Enhances Nitric Oxide Production in Trabecular Meshwork Cells.
Jae Woo Kim
Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr
Abstract
PURPOSE
This study investigated the role of ascorbic acid on the production of nitric oxide (NO) in the trabecular meshwork (TM) cells. METHODS: After primarily cultured human TM cells were exposed to 1, 10, and 100 micrometer of L-ascorbic acid (LAA), with or without co-administration of 1 mM sodium nitroprusside or 100 micrometer hydrogen peroxide for 48 hr, cellular survival and NO production were measured with MTT and Griess assay, respectively. RESULTS: LAA significantly potentiated NO production in a dose-dependent manner (p< 0.05) without affecting cell viability. LAA increased cell viability after hydrogen peroxide-induced oxidative stress in a dose-dependent manner. LAA enhanced NO production in TM cells and showed a cytoprotective effect against hydrogen peroxide-induced oxidative stress. CONCLUSIONS: LAA might be involved in the regulation of trabecular outflow by enhancing NO production in TM cells.
Key Words: Ascorbic acid;Hydrogen peroxide;Nitric oxide;Trabecular meshwork cell


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