| Safety of intravitreally injected ciprofloxacin in phakic rabbit eyes. |
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Sang Ha Kim, Jae Hong Kim, Kyung Shin Cho, Jung Sik Kwak |
1Department of Ophthalmology, School of Medicine, Kyungpook National University, Taegu, Korea.
2Department of Ophthalmology, Maryknoll Hospital, Pusan, Korea.
3Department of Pathology, School of Medicine, Kyungpook National University, Taegu, Korea. |
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| Abstract |
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This study was designed to determine the maximal safe drug concentration of intravitreal ciprofloxacin in phakic rabbit eyes. Twenty-two eyes of New Zealand pigmented rabbits received midvitreal ciprofloxacin of 100, 200, 400, 600 or 800 microgram in BSS Plus, or BSS Plus only. Retinal toxicity was dose-dependent as determined with electroretinography, light microscopy, and transmission electron microscopy. At a dose of greater than 400 microgram, disorganization of the outer segments was a main pathological finding in transmission electron microscopy. We evaluated retinal function by measuring the electroretinograms for a graded series of flash intensities and by fitting electroretinogram b-wave amplitudes to the Naka-Rushton equation. At a dose of greater than 600 microgram, Rmax was significantly decreased and log K was significantly increased. N-value tended to decrease. A decrease of b-wave amplitudes caused by retinal toxicity could be detected very sensitively with lower luminance stimuli. Determination of retinal toxicity with lower luminance electroretinography revealed a significant decrease of b-wave amplitudes at a dose of greater than 400 microgram. We concluded that a safe dose of intravitreal ciprofloxacin in phakic rabbit eyes was 200 microgram in phakic eyes. |
| Key Words:
intravitreal ciprofloxacin;electroretinogram;Naka-Rushton equation parameters;electron microscory;retinal toxicity |
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