Korean J Ophthalmol > Volume 10(2); 1996 > Article
Korean Journal of Ophthalmology 1996;10(2):68-75.
DOI: https://doi.org/10.3341/kjo.1996.10.2.68    Published online December 30, 1996.
Effect of topically applied Na-hyaluronan on experimental corneal alkali wound healing.
Jang Hyun Chung, Hyung Joon Kim, Per Fagerholmb, Byung Chae Cho
1Department of Ophthalmology, Mokdong Hospital and Medical Research Center, Ewha Womans University, Seoul, Korea.
2St. Eriks Eye Hospital, Karolinska Institude, Stockholm, Sweden.
The effect of topically applied 1% sodium hyaluronate (Na-HA) on the healing of a standardized corneal alkali wound was studied. The healing of the epithelium, stroma, and endothelium was evaluated separately, using quantitative methods. Central corneal alkali wound was produced in one eye of the rabbits by applying a 5.5-mm round filter paper, soaked in 1 N NaOH, for 60 seconds. 1% Na-HA in the treatment group and phosphate buffered saline (PBS) in the control group were given topically 4 times per day for 2 days, 1- and 3-weeks. Epithelial and endothelial healing was assessed morphometrically from standardized photographs and micrographs, respectively. Stromal healing was determined by counting polymorphonuclear leukocytes (PMN) and keratocytes in the central and marginal wound areas. A positive healing influence was observed in the epithelium. In stromal healing, 1% Na-HA treated corneas showed less PMNs and more keratocytes than the control group, suggesting that topically applied 1% Na-HA may suppress the stromal PMN infiltration and enhance the keratocyte repopulation during corneal alkali wound healing. However, no significant difference was found in morphometric evaluation of endothelial healing between the two groups.
Key Words: hyaluronan;cornea;alkali wound;epithelium;strorna;keratocyte;PMN;endothelium;rabbit

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