Korean J Ophthalmol > Volume 36(4); 2022 > Article
Korean Journal of Ophthalmology 2022;36(4):318-325.
DOI: https://doi.org/10.3341/kjo.2022.0012    Published online June 15, 2022.
Impact of Lacrimal Gland Extraction on the Contralateral Eye in an Animal Model for Dry Eye Disease
Minha Kim1, So Young Kim2,3, Ji Won Jeon1, Hyung Keun Lee1,2
1Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea
2Severance Institute for Vascular and Metabolic Research, Yonsei University College of Medicine, Seoul, Korea
3Institute of Biomedical Research, Yonsei University College of Medicine, Seoul, Korea
Correspondence:  Hyung Keun Lee, Tel: 82-2-2019-3440, Fax: 82-2-3463-1049, 
Email: shadik@yuhs.ac
Received: 6 February 2022   • Revised: 19 April 2022   • Accepted: 21 April 2022
Abstract
Purpose
Although there is still no consensus on the best animal model for dry eye disease research, a model based on lacrimal gland extraction (LGE) model is widely used. In this study, we aimed to investigate the histopathological changes taking place on the contralateral eye after unilateral LGE to determine whether it is useful as a control.
Methods
Seven-week-old male C57BL/6 mice were divided into naive control, environmental chamber model, and LGE groups. Corneal fluorescein staining was scored to quantify the severity of damage. Morphological changes in the cornea, conjunctiva, and lacrimal gland (LG) were determined by hematoxylin and eosin staining and compared to those on naive control animals.
Results
Compared to naive subjects, the unilateral LGE model showed enhanced corneal erosion scores and loss of conjunctival goblet cells, not only on the ipsilateral but also on the contralateral side. These changes in the ocular surface became more pronounced in a time-dependent manner. Furthermore, loss of LG acinar cells and leukocyte infiltration were detected in the contralateral LGs of the LGE model.
Conclusions
Considering the changes observed in the ocular surface and LGs, the contralateral side of the LGE model may not offer proper control conditions for the experimental comparison of the effects of dry eye disease in vivo. There may be regulatory feedback or crosstalk system between both eyes activated in response to LGE.
Key Words: Animal models, Dry eye syndromes, Lacrimal gland
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